Journal: Cell Communication and Signaling : CCS

Article Title: Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration

doi: 10.1186/s12964-025-02261-5

Figure Lengend Snippet: Decreased IFN-I signaling was observed in Trem2 -deficient microglia. ( A , B ) Gene set enrichment analysis (GSEA) using gene sets from Gene Ontology Biological Process (GOBP) ( A ) and Kyoto Encyclopedia of Genes and Genomes (KEGG) ( B ) databases shows decreased expression of genes associated with “type I interferon (IFN-I) signaling” (at P18) and “phagosome” (at P21) in Trem2 −/− :rd10 retina. The criteria for GSEA are set at|NES| > 1, p < 0.05 and FDR q < 0.25. ( C ) A heatmap generated to visualize the expression of interferon-stimulated genes (ISGs) in rd10 and Trem2 −/− :rd10 retina. ( D - F ) Relative mRNA expression of selected ISGs, including Cxcl10 ( D ), Ifitm3 ( E ), Ifnar1 ( F ), in purified Trem2 −/− :rd10 and rd10 microglia. n = 3, unpaired Students’ t test. ( G ) Representative images of IBA1 and IFITM3 co-staining in Trem2 −/− :rd10 and rd10 retina at P15, P18, P21 and P30. The white arrows and white squares indicate double positive cells. ( H ) Quantification of IFITM3/IBA1 fluorescence intensity ratio in IBA1 + cells indicates decreased IFITM3 expression in Trem2 −/− :rd10 microglia. n = 3, unpaired Students’ t test. Data are curated and presented as described in Fig.

Article Snippet: IFN-α/β receptor I (IFNAR1) neutralizing antibody (#16-5945-85, ThermoFisher Scientific, CA, USA) [ ] and control IgG antibody (#16-4714-82, ThermoFisher) were administrated by intravitreal injection of 2 μL at the day when MNU was injected.

Techniques: Expressing, Generated, Purification, Staining, Fluorescence

Journal: Cell Communication and Signaling : CCS

Article Title: Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration

doi: 10.1186/s12964-025-02261-5

Figure Lengend Snippet: IFN-I signaling was activated in mouse retina with microglia-specific Trem2 overexpression. ( A ) Relative mRNA expression of Trem2 in Tmem119 CreERT2 : Rosa26 CAG − LSL−Trem2 microglia with and without tamoxifen administration. n = 3, unpaired Students’ t test. ( B , C ) Representative images and quantification of TREM2 protein expression in microglia of two groups. The white arrows indicate TREM2-expression cells. n = 3, unpaired Students’ t test. ( D , E ) Representative images and quantification of IFITM3 expression in Vehicle + Control, Vehicle + MNU-3d and Tamoxifen + MNU-3d group. The white arrows indicate double positive cells. n = 3, one-way ANOVA with Tukey’s test. ( F , G ) Representative images and quantification of Phospho-STAT1 (p-STAT1) expression in the above three groups. The white arrows indicate double positive cells. n = 3, one‐way ANOVA with Tukey’s test. ( H - J ) Relative mRNA expression of selected ISGs, including Cxcl10 ( H ), Ifitm3 ( I ), Ifnar1 ( J ), in purified microglia from Vehicle + Control, Vehicle + MNU-3d and Tamoxifen + MNU-3d mouse retinas. n = 3, one‐way ANOVA with Tukey’s test. Data are curated and presented as described in Fig.

Article Snippet: IFN-α/β receptor I (IFNAR1) neutralizing antibody (#16-5945-85, ThermoFisher Scientific, CA, USA) [ ] and control IgG antibody (#16-4714-82, ThermoFisher) were administrated by intravitreal injection of 2 μL at the day when MNU was injected.

Techniques: Over Expression, Expressing, Control, Purification